high molecular weight hmw adiponectin  (ALPCO)

Journal: Journal of the Endocrine Society

Article Title: Transgender Women With Suppressed Testosterone Display Lower Burden of Coronary Disease Than Matched Cisgender Men

doi: 10.1210/jendso/bvae120

Figure Lengend Snippet: Biomarker concentrations

Article Snippet: Soluble CD14 (sCD14, R and D Systems catalog No. DC140, RRID:AB_3095885), soluble CD163 (sCD163, R and D Systems catalog No. DC1630, RRID:AB_3096052), interleukin-6 (IL-6, R and D Systems catalog No. HS600C, RRID:AB_2893335), fatty acid-binding protein-4 (FABP-4, (ImmunoDiagnostics catalog No. 31030, RRID:AB_2933962), high-molecular-weight adiponectin (R and D Systems catalog No. DRP300, RRID:AB_2783020), insulin (R and D Systems catalog No. DINS00, RRID:AB_3073852), endothelin-1 (ET-1, R and D Systems catalog No. DET100, RRID:AB_3099470), and vascular cell adhesion molecule-1 (VCAM-1, R and D Systems catalog No. DVC00, RRID:AB_2941366) were analyzed by Quantikine ELISA (enzyme-linked immunosorbent assay), and soluble tumor necrosis factor receptor type (sTNFR, R and D Systems catalog No. LUCAM726, RRID:AB_3099473) I/II, interleukin-8 (IL-8, R and D Systems catalog No. DY208, RRID:AB_2892143), and plasminogen activator inhibitor-1 (PAI-1, R and D Systems catalog No. LOBM1786, RRID:AB_3099472) were measured by Luminex assay.

Techniques: Biomarker Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Immunomodulatory Role of an Ayurvedic Formulation on Imbalanced Immunometabolics during Inflammatory Responses of Obesity and Prediabetic Disease

doi: 10.1155/2013/795072

Figure Lengend Snippet: Kal-1 rectifies the hormonal imbalance in mice fed on high-fat high-sugar diets. (a) Effect of Kal-1 on resistin levels in high-fat high-sugar fed mice at week 15. (b) Effect of Kal-1 on leptin levels in high-fat high-sugar fed mice at week 15. (c) Effect of Kal-1 on high-molecular-weight adiponectin levels in high-fat high-sugar fed mice at week 15. All doses (5, 20, and 75 μ L) of Kal-1 were supplemented along with HFHSD. LF: low fat control, HF: high-fat high-sugar control. All the values represent mean ± SEM from five animals.

Article Snippet: Leptin and resistin levels were measured using commercially available radioimmunoassay kits (Quantikine, mouse leptin and mouse resistin, immunoassay, R&D, Minneapolis, MN, USA) and high-molecular-weight adiponectin (ALPCO, adiponectin mouse total, HMW, Salem, NH, USA).

Techniques: Molecular Weight

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Immunomodulatory Role of an Ayurvedic Formulation on Imbalanced Immunometabolics during Inflammatory Responses of Obesity and Prediabetic Disease

doi: 10.1155/2013/795072

Figure Lengend Snippet: Treatment of Kal-1 brings back the levels of pro- and antihormonal levels to normal which were showing metabolic disturbed contour in high-fat high-sugar fed mice at weeks 22, 26, and 30. (a) Effect of Kal-1 treatment on leptin profile in high-fat high-sugar diet fed mice at weeks 22, 26, and 30. (b) Effect of Kal-1 treatment on high-molecular-weight adiponectin profile in high-fat high-sugar diet fed mice at weeks 22, 26, and 30. Treatment with all doses (5, 20, and 75 μ L) of Kal-1 was started only after 21 weeks (induction period) along with high-fat high-sugar diet. LF: low-fat control, HF: high-fat high-sugar control. All the values represent mean ± SEM from five animals.

Article Snippet: Leptin and resistin levels were measured using commercially available radioimmunoassay kits (Quantikine, mouse leptin and mouse resistin, immunoassay, R&D, Minneapolis, MN, USA) and high-molecular-weight adiponectin (ALPCO, adiponectin mouse total, HMW, Salem, NH, USA).

Techniques: Molecular Weight

Journal: iScience

Article Title: Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors

doi: 10.1016/j.isci.2022.105750

Figure Lengend Snippet:

Article Snippet: High Molecular Weight and Total Adiponectin ELISA , ALPCO , Cat# 47-ADPMS-E01.

Techniques: Recombinant, High Molecular Weight, Enzyme-linked Immunosorbent Assay, Software, Modification

Journal: Nature Communications

Article Title: Genomic loci mispositioning in Tmem120a knockout mice yields latent lipodystrophy

doi: 10.1038/s41467-021-27869-2

Figure Lengend Snippet: Glucose tolerance test in control and Ad-Tmem120a −/− females on HFD ( a ) and LFD ( b ), fasting for 16 h, and quantification of the area under the curve (AUC) ( c ), panels shown as mean ± SEM, control LFD n = 9, Ad-Tmem120a −/− LFD n = 8, control HFD n = 9, Ad-Tmem120a −/− n = 9. d Insulin tolerance test in control and Ad-Tmem120a −/− females on LFD vs HFD, fasting for 6 h, shown as mean ± SEM, control LFD n = 7, Ad-Tmem120a −/− LFD n = 6, control HFD n = 9, Ad-Tmem120a −/− n = 6. Blood biochemistry. Fasting serum insulin ( e ), fasting leptin ( f ), high molecular weight (HMW) adiponectin ( g ), NEFA—nonestrified fatty acids ( h ), and triglycerides levels ( i ) in control and Ad-Tmem120a −/− females on LFD vs HFD, fasting for 16 h, shown as mean ± SEM, control LFD n = 4, Ad-Tmem120a −/− LFD n = 3, control HFD n = 4, Ad-Tmem120a −/− n = 4. j Representative images of haematoxylin and eosin-stained liver sections from control and Ad-Tmem120a −/− female mice on HFD, scale bars: 40 µm. k Quantification of lipid droplet size in liver form control and Ad-Tmem120a −/− females on HFD, the sizes of the lipid droplets were assigned to size bins and are shown as frequency, area in µm2, shown as mean ± SEM, control HFD n = 9, Ad-Tmem120a −/− n = 9. l Mean daily water intake by control and Ad-Tmem120a −/− females after 10 weeks on HFD, shown as mean ± SEM, (for each group n = 4), P values: by unpaired, two-sided Student’s t test. Source data are provided as a Source Data file.

Article Snippet: High Molecular Weight (HMW) Adiponectin ELISA (ALPCO, cat. 47-ADPMS-E01, RRID:AB_2801466) was used according to manufacturer instruction.

Techniques: Molecular Weight, Staining